Agrobacterium tumefaciens Genetic Engineering
根瘤农杆菌基因工程
作者: Chai ZhijianZhang FangHuang YuanyuanZhang ChunxiangSyngenta Biotechnology China Co.Ltd
刊名: Molecular Plant Breeding, 2016(01), pp.92-97
中文刊名: 分子植物育种, 2016(01), pp.92-97
来源数据库: CJFDTEMP_U
DOI: CNKI:SUN:FZZW.0.2016-01-020
关键词: AgrobacteriumAgrobacterium tumefaciensAgrobacterium tumefaciens genetic engineeringPlant genetic engineering
中文关键词: 土壤农杆菌根瘤农杆菌根瘤农杆菌基因工程植物基因工程
英文摘要: Agrobacterium tumefaciens is a gram-negative plant pathogenic bacterium found in soil. The tumor-inducing Ti plasmid of Agrobacterium tumefaciens carries vir genes that express Vir proteins, which help to transfer T-DNA into plant cells. T-DNA is later integrated into the plant genome, and finally causes tumors in plant. Based on its gene transfer ability, modified Agrobacterium tumefaciens strains have been used widely in plant genetic engineering field. This article reviews the genetic engineering of Agrobacterium tumefaciens for its use in plant engineering work, and intends to provide thoughts for further Agrobacterium tumefaciens modification work in the plant engineering field. These modifications include T-DNA disarming, usage of binary vector system, and Agrobacterium tumefaciens...
中文摘要: 根瘤农杆菌(Agrobacterium tumefaciens)是一类存在于土壤中的革兰氏阴性植物病原菌。根瘤农杆菌通过Ti质粒上的一系列vir基因表达毒性Vir蛋白将其自身的转移DNA(T-DNA)转移整合到植物基因组,进而达到侵染植物的目的。基于这一基因转移功能,改造后的根瘤农杆菌被广泛应用于植物基因工程。本文重点回顾了以在植物基因工程领域应用根瘤农杆菌为目的对根瘤农杆菌基因组进行的遗传改造。这些改造包括T-DNA区的卸甲,双元载体的应用,其他基因组基因的敲除等。通过回顾和展望为进一步在植物基因工程中应用根瘤农杆菌提供新的研究思路。
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