CHK1 inhibitor SCH900776 suppresses proliferation and migration of U251 cells
CHK1抑制剂SCH900776抑制胶质瘤细胞U251的增殖及迁移
作者: LI Zhao-junLI Rong-yaoWANG RuFEI Hong-rongWANG Feng-zeSchool of Pharmaceutical Sciences,Taishan Medical UniversitySchool of Life Sciences,Taishan Medical University
刊名: Chinese Journal of Pathophysiology, 2018(02), pp.200-205
中文刊名: 中国病理生理杂志, 2018(02), pp.200-205
来源数据库: CJFDTEMP_U
关键词: Checkpoint kinase 1SCH900776Cell proliferationCell migrationGlioma
中文关键词: 细胞周期检测点激酶1SCH900776细胞增殖细胞迁移胶质瘤
英文摘要: AIM: To investigate the effect of SCH900776,an inhibitor of checkpoint kinase 1( CHK1),on the proliferation and migration abilities of human glioma U251 cells. METHODS: The cell viability was detected by MTT assay,and cell proliferation was determined by cell colony formation assay. Cell cycle distribution was analyzed by flow cytometry. Wound healing assay was used to determine the cell migration ability. The protein levels were determined by Western blot. RESULTS: SCH900776 inhibited the growth of U251 cells in a dose-dependent manner( P < 0. 05).SCH900776 treatment substantially induced U251 cell cycle arrest in S and G2/M phases by decreasing the level of cell division cycle protein 2( Cdc2) and p-Cdc2. Moreover,SCH900776 inhibited the cell migration. Western blot results indicated...
中文摘要: 目的:本研究以胶质瘤细胞U251为实验用细胞株,探讨细胞周期检测点激酶1(CHK1)抑制剂SCH900776对其增殖及迁移的影响。方法:MTT法和细胞集落形成实验观察SCH900776对细胞增殖的影响;流式细胞术分析SCH900776对细胞周期分布的影响;划痕实验观察SCH900776对细胞迁移的影响;Western blot实验检测相关蛋白的表达水平。结果:SCH900776能够明显抑制U251细胞的增殖,诱导细胞发生S期或G2/M期阻滞,同时下调细胞分裂周期蛋白2(Cdc2)和p-Cdc2的水平;SCH900776对细胞迁移表现出明显的抑制作用;Western blot结果表明,SCH900776可升高p38丝裂原活化蛋白激酶(MAPK)的磷酸化水平,同时抑制蛋白激酶B(Akt)的磷酸化激活。结论:CHK1抑制剂SCH900776能够抑制胶质瘤细胞U251的增殖与迁移,其机制可能与其激活p38MAPK和抑制Akt活性相关。
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关键词翻译
关键词翻译
  • U251 SCH900776
  • CHK1 SCH900776
  • 细胞增殖 细胞迁移