A practical guide for functional transcriptomics: a case study in RNA interference and qPCR to understand the explosive chemistry of Brachinus bombardier beetles (Coleoptera: Carabidae).
作者: Gill, A. S.Gee, M.Will, K.
作者单位: Department of Environmental Science and Policy Management, University of California, Berkeley, California, USA.
英文丛书称: Transcriptomics in entomological research
出版社: CABI Publishing,   2020
ISBN: 9781789243130
来源数据库: Centre for Agriculture and Biosciences International
DOI: 10.1079/9781789243130.0181
原始语种摘要: Transcriptomics stands to benefit functional studies by providing a direct path to developing genome-wide toolboxes in almost any insect system. This chapter aims to communicate the best practices in insect functional transcriptomics, defined as the use of transcriptomic data to identify and validate genes that express phenotypes of interest. A workflow is described for identifying candidate genes based on de novo transcriptome assemblies, functionally validating candidates using RNA interference (RNAi), and quantifying knockdown expression levels with quantitative PCR (qPCR). The chapter emphasizes 'universal' steps that could apply to any transcriptome study once an assembled, annotated transcriptome is obtained. These include design and delivery of short interfering RNA (siRNA) used...
全文获取路径: CABI 
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关键词翻译
关键词翻译
  • functional 功能的
  • Coleoptera 鞘翅目
  • RNA ROYAL NEPAL AIRLINES CORP.
  • bombardier 轰炸员
  • knockdown 击倒
  • interference 干涉
  • expression 表示
  • interfering 干涉
  • study 学习
  • chapter