Rate- and depth-dependent nanomechanical behavior of individual living Chinese hamster ovary cells probed by atomic force microscopy
作者: Minhua ZhaoCharudharshini SrinivasanDiane J. BurgessBryan D. Huey
作者单位: 1Institute of Materials Science , University of Connecticut , Storrs , Connecticut 06269
2 School of Pharmacy , University of Connecticut , Storrs , Connecticut 06269
3 Institute of Materials Science , University of Connecticut , Storrs , Connecticut 06269
刊名: Journal of Materials Research, 2006, Vol.21 (8), pp.1906-1912
来源数据库: Cambridge University Press Journal
DOI: 10.1557/jmr.2006.0233
原始语种摘要: A single elastic modulus is not sufficient for describing the mechanical behavior of a living cell due to its viscoelastic nature and heterogeneity beneath the membrane. In this paper, the nanoscale elastic and viscoelastic behavior of individual living Chinese hamster ovary (CHO-K1) cells in a physiological environment were probed by atomic force microscopy (AFM) indentations at various loading rates. Based on Hertzian fits of the force–distance curves, the apparent elastic modulus of the cells was determined and found to be a function of the loading rate as well as the indentation depth. Notably, contributions from the substrate were negligible up to 50% of the cell thickness. For increased indentation rates and depths, healthy spindle-shaped CHO-K1 cells were found to exhibit an...
全文获取路径: Cambridge U Press 
分享到:
来源刊物:
影响因子:1.713 (2012)

×
关键词翻译
关键词翻译
  • atomic 原子的
  • microscopy 显微镜检查
  • behavior 行为
  • loading 加负载
  • viscoelastic 粘弹性的
  • modulus 系数
  • cells 麻风细胞
  • force 
  • indentation 锯齿痕
  • thickness 厚度