Evaluation of ELISA and haemagglutination inhibition as screening tests in serosurveillance for H5/H7 avian influenza in commercial chicken flocks
作者: M. E. ArnoldM. J. SlomkaA. C. BreedC. K. HjulsagerS. Pritz-VerschurenS. Venema-KemperR. J. BouwstraR. TrebbienS. ZohariV. CeerazL. E. LarsenR. J. ManvellG. KochI. H. Brown
作者单位: 11 Department of Epidemiological Sciences (Biomathematics and Risk Research) , Animal and Plant Health Agency (APHA) , The Elms , College Road , Sutton Bonington , Loughborough LE12 5RB , UK
2 2 European Union Reference Laboratory for Avian Influenza and Newcastle Disease , APHA-Weybridge , Woodham Lane , Addlestone KT15 3NB , UK
3 3 Avian Virology Workgroup , Virology Department , APHA-Weybridge , Woodham Lane, Addlestone KT15 3NB , UK
4 4 Department of Epidemiological Sciences (Epidemiology) , APHA , Kendal Road , Harlescott , Shrewsbury SY1 4HD , UK
5 5 National Veterinary Institute , Technical University of Denmark (DTU) , Kemitorvet, Building 204, 2800 Lyngby , Denmark
6 6 Wageningen Bioveterinary Research , Houtribweg 39, 8221 RA Lelystad , The Netherlands
7 7 National Veterinary Institute (SVA) , Ulls väg 2B, Uppsala , Sweden
刊名: Epidemiology and Infection, 2018, Vol.146 (3), pp.306-313
来源数据库: Cambridge University Press Journal
DOI: 10.1017/S0950268817002898
原始语种摘要: Abstract Avian influenza virus (AIV) subtypes H5 and H7 can infect poultry causing low pathogenicity (LP) AI, but these LPAIVs may mutate to highly pathogenic AIV in chickens or turkeys causing high mortality, hence H5/H7 subtypes demand statutory intervention. Serological surveillance in the European Union provides evidence of H5/H7 AIV exposure in apparently healthy poultry. To identify the most sensitive screening method as the first step in an algorithm to provide evidence of H5/H7 AIV infection, the standard approach of H5/H7 antibody testing by haemagglutination inhibition (HI) was compared with an ELISA, which detects antibodies to all subtypes. Sera ( n = 1055) from 74 commercial chicken flocks were tested by both methods. A Bayesian approach served to estimate diagnostic test...
全文获取路径: Cambridge U Press  (合作)
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影响因子:2.867 (2012)

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关键词翻译
关键词翻译
  • flocks 絮凝物
  • chicken 小鸡
  • influenza 列性感冒
  • avian 鸟的
  • ELISA 酶联免疫吸收分析
  • pathogenicity 病原性
  • AIV Accumulator Isolation Valve
  • screening 筛分
  • commercial 商业的
  • infected 被感染