A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus
作者: Gokhan BahceciogluNesrin HasirciBahar BilgenVasif Hasirci
作者单位: 1BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Ankara, Turkey
2Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
3Department of Biotechnology, Middle East Technical University, Ankara, Turkey
4Department of Chemistry, Middle East Technical University, Ankara, Turkey
5Providence VA Medical Center, Providence, RI, United States of America
6Department of Orthopaedics, The Warren Alpert Medical School of Brown University and Rhode Island Hospital, Providence, RI, United States of America
7Department of Medical Engineering, Acıbadem Mehmet Ali Aydınlar University, Istanbul, Turkey
刊名: Biofabrication, 2019, Vol.11 (2)
来源数据库: Institute of Physics Journal
DOI: 10.1088/1758-5090/aaf707
原始语种摘要: Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion. Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively. After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold ( p < 0.001), while GelMA enhanced collagen production by about 50 fold ( p < 0.001). In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs...
全文获取路径: IOP  (合作)
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来源刊物:
影响因子:3.705 (2012)

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关键词翻译
关键词翻译
  • hydrogel 水凝胶
  • meniscus 弯月面
  • constructs 建筑图纸生成系统
  • biochemical 生物化学的
  • collagen 胶骨硬蛋白质
  • scaffold 脚手架
  • PCL Packet Control Layer
  • gelatin 
  • portion 部分
  • fibrocartilage 纤维软骨