One-Step Real-Time Reverse Transcription–PCR for the Detection of Turkey Reoviruses
作者: Sunil K. MorHarsha VermaAschalew Z. BekeleTamer A. SharafeldinRobert E. PorterSagar M. Goyal
刊名: Avian Diseases, 2014, Vol.58 (3), pp.404-407
来源数据库: BioOne Complete
DOI: 10.1637/10779-012314-Reg.1
关键词: turkey reovirusreal-time RT-PCRTaqmanS4 gene
原始语种摘要: During late 2010 and early 2011, an unusual problem of lameness and swollen hock joints in commercial turkeys was reported in the upper Midwest, which continues to this day. The disease caused substantial economic losses to turkey producers. Reovirus was isolated from tendons and joint fluids of lame turkeys submitted to the Minnesota Veterinary Diagnostic Laboratory. This study was undertaken to develop a TaqMan real-time reverse transcription-PCR (rRT-PCR) assay for the early detection of turkey reoviruses (both enteric and lameness strains). A primer probe set was designed from the conserved region of the S4 segment of the turkey reovirus genome. The newly developed rRT-PCR was specific for the detection of turkey reoviruses. The detection limit of this assay was 10 genome copies per...

  • 关节炎 探测
  • reovirus 呼肠孤病毒
  • PCR Page Control Register
  • turkey 火鸡
  • lameness 瘸子
  • detection 探测
  • arthritis 探测
  • indicating 指示
  • infectious 传染性的
  • submitted 提交的
  • early