Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish ( Danio rerio ) Embryos at the Dome Stage Using Suppression Subtractive Hybridization<sup>1</sup>
作者: Daji LuoWei HuShangping ChenYi XiaoYonghua SunZuoyan Zhu
刊名: Biology of Reproduction, 2009, Vol.80 (4), pp.674-684
来源数据库: BioOne Complete
DOI: 10.1095/biolreprod.108.074203
关键词: embryodifferentially expressed genesreprogrammingsomatic cell nuclear transfersuppression subtractive hybridization
原始语种摘要: Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on...
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影响因子:4.027 (2012)

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关键词翻译
关键词翻译
  • expressed 榨出的
  • sup 吃晚饭
  • reprogramming 改编程序
  • somatic 躯体的
  • subtractive 减去
  • nuclear 原子核的
  • annotation 注解
  • understanding 理解
  • importance 重要性
  • regulation