Reconstituted fusion liposomes for gene transfer in vitro and in vivo
作者: Ken SuzukiHiroshi NakashimaYoshiki SawaRyuichi MorishitaHikaru MatsudaYasufumi Kaneda
刊名: Gene Therapy and Regulation, 2000, Vol.1 (1), pp.65 -77
来源数据库: Brill期刊
DOI: 10.1163/156855800744539
原始语种摘要: Using UV-inactivated whole HVJ (Hemagglutinating Virus of Japan; Sendai virus), we have developed HVJ-liposomes that are efficient in vitro and in vivo gene delivery vehicles based on cell fusion properties of the Sendai virus. However, all the proteins and the genome of HVJ remain within the HVJ-liposomes, although replication of the viral genome is severely impaired by prior UV-irradiation of HVJ particles. To construct more complete synthetic vehicles, we developed reconstituted fusion liposomes. Fusion proteins F1 and HN of HVJ were extracted by mild lysis of the viral particles and purified by ion-exchange column chromatography. Purified viral fusion proteins were inserted into liposome membranes by detergent-solubilization and dialysis to construct the reconstituted fusion...
全文获取路径: Brill Press 

  • fusion 融解
  • deliver 输送
  • vitro 体外
  • isothiocyanate 异硫氰酸盐
  • luciferase 荧光素酶
  • developed 开发
  • viral 病毒性的
  • muscle 肌肉
  • amniotic 羊膜的
  • vortexing 涡流