TLC-densitometric analysis of allantoin in Symphytum officinale L. roots
作者: Katarzyna KimelMagdalena ZienkiewiczBarbara Sparzak-StefanowskaMirosława Krauze-Baranowska
作者单位: 1Medical University of Gdańsk , Department of Pharmacognosy with the Medicinal Plants Garden , Gdańsk , Poland
刊名: Acta Pharmaceutica, 2020, Vol.70 (1), pp.101-110
来源数据库: De Gruyter Journal
DOI: 10.2478/acph-2020-0014
关键词: AllantoinSymphytum officinaleDensitometryTLCHPLC
原始语种摘要: Abstract A TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 ( V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/water 80:20 ( V/V ) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer,...
全文获取路径: De Gruyter 

  • 显影 测定
  • allantoin 尿囊素
  • roots 麻根
  • analysis 分析
  • densitometric 密度计的
  • acetonitrile 乙腈
  • agreement 协议
  • quantification 量化
  • determination 测定
  • developing 测定
  • detection 探测