作者: Sibel YilmazNermin Gozukirmizi
作者单位: 1Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul, Turkey
刊名: Biotechnology & Biotechnological Equipment, 2013, Vol.27 (6), pp.4227-4230
来源数据库: Diagnosis Press Journal
DOI: 10.5504/BBEQ.2013.0076
关键词: BARE1Hordeum vulgare L.IRAPretrotransposontissue culture
原始语种摘要: In this study, tissue-culture-induced BARE1 (BArley Retrotransposable Element-1) retrotransposon movements were investigated in barley calli and regenerated shoots, using the Inter-Retrotransposon Amplified Polymorphism (IRAP) technique. IRAP analysis was carried out on 30-, 60-, 90-day-old calli and shoots regenerated from them. Samples from calli and regenerated shoots originating from same embryo were accepted as a group. Two sample groups originating from two different embryos were analyzed. DNAs from three mature embryos were used as control samples. The IRAP analysis of the control samples showed identical IRAP profiles of 12 bands for all three mature embryos, suggesting a lack of natural polymorphism between the tested individuals. The IRAP profiles of the two analyzed groups were...
全文获取路径: Diagnosis Press  (合作)
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关键词翻译
关键词翻译
  • regenerated 再生的
  • bands 同心带
  • conditions 条件式
  • cultivation 栽培
  • polymorphism 多晶形
  • originating 发信
  • barley 大麦
  • group 
  • possibly 无论如何
  • callus 骨痂