作者: Sha WangFengmei YangLijun JiuWen ZhangWene ZhangZhiguo TianFei Wang
作者单位: 1Northwest A&F University, College of Forestry, Yangling, Shaanxi, P.R. China
2Northwest A&F University, College of Horticulture, Yangling, Shaanxi, P.R. China
3Guizhou University, College of Agriculture, Guiyang, Guizhou, P. R. China
4Changzhou University, College of Art, Changzhou, Jiangsu, P.R. China
刊名: Biotechnology & Biotechnological Equipment, 2013, Vol.27 (6), pp.4243-4247
来源数据库: Diagnosis Press Journal
DOI: 10.5504/BBEQ.2013.0092
关键词: direct somatic embryogenesishistologyindirect somatic embryogenesismicropropagationplant growth regulators (PGRs)
原始语种摘要: A system of plant regeneration from leaf explants of Muscari armeniacum via somatic embryogenesis was established. The process of somatic embryogenesis was studied, and histological observation of this process was made. The results showed that: somatic embryos could be obtained from direct embryogenesis or indirect embryogenesis, and less abnormal embryos were achieved through the direct approach, i.e. direct somatic embryos were more suitable for plant propagation and genetic transformation; MS basal medium containing 0.5 mg∙L-1 2,4-D and 0.1 mg∙L-1 TDZ showed a high frequency of indirect somatic embryo production, while MS basal medium supplement with 0.1 mg∙L-1 6-BA and 0.1 mg∙L-1 TDZ exhibited a high frequency of direct somatic embryogenesis...
全文获取路径: Diagnosis Press  (合作)
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关键词翻译
关键词翻译
  • somatic 躯体的
  • embryogenesis 胚发生
  • plantlet 小植物
  • indirect 间接的
  • genetic 遗传的
  • observation 观测
  • process 过程
  • less 更少的
  • shaped 定形的
  • established 确定