Use of the comet-FISH assay to compare DNA damage and repair in p53 and hTERT genes following ionizing radiation.
作者: Declan J McKennaBernadette A DohertyC Stephen DownesStephanie R McKeownValerie J McKelvey-Martin
刊名: PLoS ONE, 2017, Vol.7 (11)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0049364
原始语种摘要: The alkaline single cell gel electrophoresis (comet) assay can be combined with fluorescent in situ hybridisation (FISH) methodology in order to investigate the localisation of specific gene domains within an individual cell. The number and position of the fluorescent signal(s) provides information about the relative damage and subsequent repair that is occurring in the targeted gene domain(s). In this study, we have optimised the comet-FISH assay to detect and compare DNA damage and repair in the p53 and hTERT gene regions of bladder cancer cell-lines RT4 and RT112, normal fibroblasts and Cockayne Syndrome (CS) fibroblasts following γ-radiation. Cells were exposed to 5Gy γ-radiation and repair followed for up to 60 minutes. At each repair time-point, the number and location of p53 and...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • comet 彗星
  • repair 修理
  • assay 试金
  • radiation 辐射
  • region 地域
  • fluorescent 萤光的
  • DNA Deoxyribonucleic Acid
  • relative 相对的
  • compare 比较
  • detect 探测