Lipopolysaccharides Shapes the Human Wharton's Jelly-Derived Mesenchymal Stem Cells in Vitro
作者: Ya-Bo MeiWei-Qin ZhouXiao-Ying ZhangXiu-Juan WeiZhi-Chun Feng
刊名: Cellular Physiology and Biochemistry, 2013, Vol.32 (2), pp.390-401
来源数据库: Directory of Open Access Journals
DOI: 10.1159/000354446
关键词: LipopolysaccharidesMesenchymal stem cellsToll-like receptor 4Wharton’s jelly
原始语种摘要: Background: Although the expression of toll-like receptors (TLRs) on different types of human mesenchymal stem cells (hMSCs) has recently been reported, controversy remains regarding the presence of TLR4 as well as its engagement and impact on human Wharton's jelly-derived MSCs (hWJ-MSCs). Methods: In the present study, the expression and role of TLR4 in hWJ-MSCs was investigated using a model of lipopolysaccharide (LPS). Proliferation, apoptosis, and the expression of paracrine factors in hWJ-MSCs primed with LPS were analysed. Results: The expression of TLR4 was high at the RNA level but very low at the protein level. hWJ-MSCs responded to LPS stimulation and initiated a marked up-regulation of inflammatory cytokine (IL-1α, IL-1β, IL-6, and IL-8) production. Moreover, hWJ-MSCs LPS...
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  • engagement 啮合
  • cytokines 细胞浆移动
  • receptor 接受体
  • LPS Laboratory Peripheral System
  • stimulation 激励
  • dioxygenase 加双氧酶
  • primed 待发的
  • expression 表示
  • potential 
  • remains 残渣