A Method for Determining the Content of Glycoproteins in Biological Samples
作者: Yang GaoDuoduo XuHongyue LiXianling YangMingxing WangQipin Gao
作者单位: 1ilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China
2Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China
3Jilin Institute For Drug Control, Changchun 130033, Jilin, China
4Jilin Key Laborato
刊名: Molecules, 2016, Vol.21 (12)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/molecules21121625
关键词: GlycoproteinIodine substitutionICP-MSBiological sampleQuantification
原始语种摘要: The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection...
全文获取路径: DOAJ  (合作)
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影响因子:2.428 (2012)

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关键词翻译
关键词翻译
  • organs 男生殖器
  • glycoprotein 糖蛋白
  • purified 净化的
  • amount 总计
  • substitution 同位素置换
  • sensitive 灵敏的
  • iodine 
  • accurate 精确的
  • administration 管理
  • biological 生物学的