On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.
作者: Se Hee LeeJae Ha LeeHo Won LeeYang-Hoon KimOk Chan JeongJi-Young Ahn
刊名: PLoS ONE, 2017, Vol.11 (7)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0159777
原始语种摘要: We fabricated droplet-based microfluidic platform for copolymerizable microspheres with acrydite modified DNA probe. The copolymerizable 3-D polyacrylamide microspheres were successfully produced from microcontinuous-flow synthesis with on-channel solidification. DNA copolymerization activity, surface presentation and thermostability were assessed by using fluorescent labeled complementary probe. The binding performance was only visible on the surface area of oligo-microspheres. We show that the resulting oligo-microspheres can be directly integrated into a streamlined microsphere-PCR protocol for amplifying ssDNA. Our microspheres could be utilized as a potential material for ssDNA analysis such as DNA microarray and automatic DNA SELEX process.
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • ssDNA 单链 DNA
  • Amplification 放大
  • fluorescent 萤光的
  • complementary 补的
  • amplifying 放大
  • streamlined 吝形的
  • solidification 凝固
  • probe 探针
  • platform 台地
  • fabricated 制造