Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
作者: Amir M. AshrafiMilan SýsEliška SedláčkováAmir Shaaban FaragVojtěch AdamJan PřibylLukáš Richtera
刊名: Sensors, 2019, Vol.19 (13)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/s19132939
关键词: Glucose oxidaseHeavy metalsAmperometric biosensorNon-competitive inhibition
原始语种摘要: The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion® layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember...
全文获取路径: DOAJ  (合作)
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影响因子:1.953 (2012)

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关键词翻译
关键词翻译
  • amperometric 测量电流的
  • heavy 重大的
  • studied 学习
  • necessary 必要的
  • carbon 
  • determination 测定
  • adsorption 吸附
  • activity 活度
  • various 千变万化的
  • metal 金属