Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
作者: Cristiane MoriwakiCassiana MazzerRúbia PazzettoGraciette Matioli
刊名: Química Nova, 2009, Vol.32 (9), pp.2360-2366
来源数据库: Directory of Open Access Journals
DOI: 10.1590/S0100-40422009000900024
关键词: CyclodextrinCGTaseBiospecific affinity chromatography
原始语种摘要: Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil. The cyclodextrins (CDs) production by CGTases was evaluated using starches from different sources. CDs were measured through the Complexation Theory and by HPLC. CGTase from Bacillus firmus strain 7B showed the best production (30 mmol/L of β-CD and 4.3 mmol/L of γ-CD), and its cultivation conditions were optimized. The maximum enzymatic activity was achieved using lung peptone, soluble starch and agitation speed of 160 rpm. Studied CGTases were shown quite interesting for the industrial production of CDs.
全文获取路径: DOAJ  (合作)

  • glycosyl 糖基
  • cyclodextrins 环糊精
  • production 生产
  • affinity 亲和力
  • purify 精制
  • performance 性能
  • chromatography 色层分离法
  • interesting 有趣的
  • cultivation 栽培
  • strain 应变