Identification of nitric oxide as an endogenous inhibitor of 26S proteasomes in vascular endothelial cells.
作者: Hongtao LiuShujie YuHua ZhangJian Xu
刊名: PLoS ONE, 2017, Vol.9 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0098486
原始语种摘要: The 26S proteasome plays a fundamental role in almost all eukaryotic cells, including vascular endothelial cells. However, it remains largely unknown how proteasome functionality is regulated in the vasculature. Endothelial nitric oxide (NO) synthase (eNOS)-derived NO is known to be essential to maintain endothelial homeostasis. The aim of the present study was to establish the connection between endothelial NO and 26S proteasome functionality in vascular endothelial cells. The 26S proteasome reporter protein levels, 26S proteasome activity, and the O-GlcNAcylation of Rpt2, a key subunit of the proteasome regulatory complex, were assayed in 26S proteasome reporter cells, human umbilical vein endothelial cells (HUVEC), and mouse aortic tissues isolated from 26S proteasome reporter and eNOS...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • cells 麻风细胞
  • vascular 维管的
  • endothelial 内皮的
  • reporter 报告生成程序
  • knockdown 击倒
  • protein 蛋白质
  • endogenous 内成的
  • derived 导生的
  • lacking 缺少的
  • inactivation 失活