Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows.
作者: Lingling WangAyat HatemUmit V CatalyurekMark MorrisonZhongtang Yu
刊名: PLoS ONE, 2017, Vol.8 (11)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0078507
原始语种摘要: The ruminal microbial community is a unique source of enzymes that underpin the conversion of cellulosic biomass. In this study, the microbial consortia adherent on solid digesta in the rumen of Jersey cattle were subjected to an activity-based metagenomic study to explore the genetic diversity of carbohydrolytic enzymes in Jersey cows, with a particular focus on cellulases and xylanases. Pyrosequencing and bioinformatic analyses of 120 carbohydrate-active fosmids identified genes encoding 575 putative Carbohydrate-Active Enzymes (CAZymes) and proteins putatively related to transcriptional regulation, transporters, and signal transduction coupled with polysaccharide degradation and metabolism. Most of these genes shared little similarity to sequences archived in databases. Genes that were...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • active 活动的
  • genetic 遗传的
  • rumen 第一胃
  • enzymes 酶类
  • contained 容纳的
  • cattle 
  • digesta 消化物
  • proteins 蛋白质
  • carried 携带
  • underpin 自下向上连接井壁