Use of Repetitive Sequences for Molecular and Cytogenetic Characterization of Avena Species from Portugal
作者: Diana TomásJoana RodriguesAna VarelaMaria Manuela VelosoWanda ViegasManuela Silva
作者单位: 1inking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
2Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
3Linking Landscape, Environm
刊名: International Journal of Molecular Sciences, 2016, Vol.17 (2)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/ijms17020203
关键词: Avena Portuguese linesRepetitive sequencesGenomic diversityMolecular markers
原始语种摘要: Genomic diversity of Portuguese accessions of Avena species—diploid A. strigosa and hexaploids A. sativa and A. sterilis—was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species—rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies—IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)—were performed. A very high homology was detected for ribosomal internal transcribed sequences (ITS1 and ITS2) between the species analyzed, although nucleolar organizing regions (NOR) fluorescent in situ hybridization (FISH) analysis revealed distinct number of Nor loci...
全文获取路径: DOAJ  (合作)
分享到:
来源刊物:
影响因子:2.464 (2012)

×
关键词翻译
关键词翻译
  • hexaploid 六倍体
  • sativa 漂白亚麻纤维卷
  • intraspecific 种内的
  • species 
  • diploid 二倍体
  • lines 线型
  • markers 时标
  • polymorphism 多晶形
  • previously 以前
  • cereal 谷物