MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4
作者: Wang LeiChen YanJiang YaDai YongBian YujunLiu Kai
刊名: BMC Cancer, 2018, Vol.18 (1), pp.1-9
来源数据库: Directory of Open Access Journals
DOI: 10.1186/s12885-018-4460-0
关键词: OsteosarcomaMulti-chemoresistanceMiR-199a-3pAK4
原始语种摘要: Abstract Background MicroRNAs (miRNAs) play vital roles in regulating various biological processes. The dysregulations of miRNAs may result in severe human diseases, including cancer. Methods We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. We further predicted the putative signal pathway involved in the miR-199a-3p-mediated OS drug-resistance. Results The AK4 gene is one of the targets of miR-199a-3p and negatively correlates with the effect of miR-199a-3p on OS drug-resistance. In addition, the activity of the NF-кB signaling pathway was...
全文获取路径: DOAJ  (合作)
影响因子:3.333 (2012)

  • targeting 导向目标
  • osteosarcoma 骨肉瘤
  • luciferase 荧光素酶
  • reporter 报告生成程序
  • addition 添加
  • further 进一步
  • involved 涉及
  • resistance 抵抗
  • detect 探测
  • various 千变万化的