Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidum
作者: Lujun HuLinlin WangWenwei LuJianxin ZhaoHao ZhangWei Chen
作者单位: 1tate Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
3State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, W
刊名: International Journal of Molecular Sciences, 2017, Vol.18 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/ijms18050883
关键词: Bifidobacterium bifidumAptamerSELEXSequence truncationColorimetric bioassay
原始语种摘要: A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. After 12 rounds of selection targeted against B. bifidum, 30 sequences were obtained and divided into seven families according to primary sequence homology and similarity of secondary structure. Four FAM (fluorescein amidite) labeled aptamer sequences from different families were selected for further characterization by flow cytometric analysis. The results reveal that the aptamer sequence CCFM641-5 demonstrated high-affinity and specificity for B. bifidum compared with the other sequences tested, and the estimated Kd value was 10.69 ± 0.89 nM. Additionally, sequence truncation experiments...
全文获取路径: DOAJ  (合作)
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影响因子:2.464 (2012)

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关键词翻译
关键词翻译
  • Selection 分选
  • primer 底层涂料
  • targeted 对准目标的
  • bioassay 生物测定
  • colorimetric 比色的
  • detect 探测
  • demonstrated 探明的储量
  • divided 分部联箱
  • sequence 次序
  • truncation