Fluorescence properties of the Na+/H+exchanger inhibitor HMA (5-(N,N-hexamethylene)amiloride) are modulated by intracellular pH
作者: V. GiansantiG. SantamariaA. TorrigliaF. ArediaA.I. ScovassiG. BottiroliA.C. Croce
刊名: European Journal of Histochemistry, 2012, Vol.56 (1), pp.e3-e3
来源数据库: Directory of Open Access Journals
DOI: 10.4081/ejh.2012.e3
关键词: ARPE19-cellsAutofluorescenceHMAIntracellular pHFluorochromeSpectroscopyImaging
原始语种摘要: HMA (5-(N,N-hexamethylene)amiloride), which belongs to a family of novel amiloride derivatives, is one of the most effective inhibitors of Na+/H+ exchangers, while uneffective against Na+ channels and Na+/Ca2+ exchangers. In this study, we provided evidence that HMA can act as a fluorescent probe. In fact, human retinal ARPE19 cells incubated with HMA show an intense bluish fluorescence in the cytoplasm when observed at microscope under conventional UV-excitation conditions. Interestingly, a prolonged observation under continuous exposure to excitation lightdoes not induce great changes in cells incubated with HMA for times up to about 5 min, while an unexpected rapid increase in fluorescence signal is observed in cells incubated for longer times. The latter phenomenon is particularly...
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影响因子:2.412 (2012)

  • amiloride 阿米洛利
  • hexamethylene 环己烷
  • cells 麻风细胞
  • properties 道具
  • induced 感应的
  • fluorescence 荧光
  • intracellular 胞内的
  • against 反对
  • exploitable 可开发的
  • unexpected 突然的