An Sp1/Sp3 binding polymorphism confers methylation protection.
作者: Yanis A BoumberYutaka KondoXuqi ChenLanlan ShenYi GuoCarmen TellezMarcos R H EstécioSaira AhmedJean-Pierre J Issa
刊名: PLoS Genetics, 2008, Vol.4 (8)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pgen.1000162
原始语种摘要: Hundreds of genes show aberrant DNA hypermethylation in cancer, yet little is known about the causes of this hypermethylation. We identified RIL as a frequent methylation target in cancer. In search for factors that influence RIL hypermethylation, we found a 12-bp polymorphic sequence around its transcription start site that creates a long allele. Pyrosequencing of homozygous tumors revealed a 2.1-fold higher methylation for the short alleles (P<0.001). Bisulfite sequencing of cancers heterozygous for RIL showed that the short alleles are 3.1-fold more methylated than the long (P<0.001). The comparison of expression levels between unmethylated long and short EBV-transformed cell lines showed no difference in expression in vivo. Electrophorectic mobility shift assay showed that the...
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影响因子:8.517 (2012)

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关键词翻译
关键词翻译
  • methylation 甲基化酌
  • binding 装订
  • allele 等位基因
  • genetic 遗传的
  • expression 表示
  • transfection 转染
  • sequencing 排序
  • polymorphism 多晶形
  • inserted 嵌入的
  • transcription 地名转写