Phenotypic Consequence of Rearranging the N Gene of RABV HEP-Flury
作者: Mingzhu MeiTeng LongQiong ZhangJing ZhaoQin TianJiaojiao PengJun LuoHe JiangYingyi LinZhixiong LinXiaofeng Guo
刊名: Viruses, 2019, Vol.11 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/v11050402
关键词: HEP-FluryRabies virusGene rearrangementNucleoprotein
原始语种摘要: Nucleoprotein (N) is a key element in rabies virus (RABV) replication. To further investigate the effect of N on RABV, we manipulated an infectious cDNA clone of the RABV HEP-Flury to rearrange the N gene from its wild-type position of 1 (N-P-M-G-L) to 2 (P-N-M-G-L), 3 (P-M-N-G-L), or 4 (P-M-G-N-L), using an approach that left the viral nucleotide sequence unaltered. Subsequently, viable viruses were recovered from each of the rearranged cDNA and examined for their gene expression levels, growth kinetics in cell culture, pathogenicity in suckling mice and protection in mice. The results showed that gene rearrangement decreased N mRNA transcription and vRNA replication. As a result, all viruses with rearranged genomes showed worse replication than that of rHEP-Flury in NA cells at a MOI of...
全文获取路径: DOAJ  (合作)
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影响因子:2.509 (2012)

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关键词翻译
关键词翻译
  • virus 病毒
  • pathogenicity 病原性
  • replication 复制
  • rearrangement 重新配置
  • rabies 狂犬病
  • attenuated 减毒的
  • suckling 乳儿
  • investigate 
  • infectious 传染性的
  • vaccine 菌苗