High performance liquid chromatography method for quantification of the N-phenylpiperazine derivative LASSBio-579 in rat plasma
作者: Daniela J. ConradoStela Maris Kuze RatesTeresa Dalla CostaCarlos Alberto Manssour FragaEliezer J. Barreiro
刊名: Química Nova, 2007, Vol.30 (8), pp.1919-1922
来源数据库: Directory of Open Access Journals
DOI: 10.1590/S0100-40422007000800023
关键词: LASSBio-579HPLCPharmacokinetics
原始语种摘要: A rapid HPLC analytical method was developed and validated for the determination of the N-phenylpiperazine derivative LASSBio-579in plasma rat. Analyses were performed using a C18 column and elution with 20 mM sodium dihydrogen phosphate monohydrate - methanol. The analyte was monitored using a photodiode array detector (257 nm). Calibration curves in spiked plasma were linear over the concentration range of 0.3-8 mg/mL with determination coefficient > 0.99. The lower limit of quantification was 0.3 mg/mL. The applicability of the HPLC method for pharmacokinetic studies was tested using plasma samples obtained after administration of LASSBio-579 to Wistar rats, showing the specificity of the method.
全文获取路径: DOAJ  (合作)

  • plasma 深绿玉髓
  • quantification 量化
  • derivative 衍生物
  • determination 测定
  • spiked 有齿的
  • phosphate 磷酸盐
  • monohydrate 一水化物
  • method 方法
  • administration 管理
  • applicability 适用性