Generation of circularly permuted fluorescent-protein-based indicators for in vitro and in vivo detection of citrate.
作者: Yuki HondaKohtaro Kirimura
刊名: PLoS ONE, 2017, Vol.8 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0064597
原始语种摘要: Indicators for citrate, particularly those applicable to its in vivo detection and quantitation, have attracted much interest in both biochemical studies and industrial applications since citrate is a key metabolic intermediate playing important roles in living cells. We generated novel fluorescence indicators for citrate by fusing the circularly permuted fluorescent protein (cpFP) and the periplasmic domain of the bacterial histidine kinase CitA, which can bind to citrate with high specificity. The ratiometric fluorescent signal change was observed with one of these cpFP-based indicators, named CF98: upon addition of citrate, the excitation peak at 504 nm increased proportionally to the decrease in the peak at 413 nm, suitable for build-in quantitative estimation of the binding compound....
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • fluorescent 萤光的
  • citrate 柠檬酸盐
  • circularly 循环地
  • vitro 体外
  • indicators 指示器
  • detection 探测
  • protein 蛋白质
  • specificity 特异性
  • demonstrated 探明的储量
  • observed 观察到的