RNAm expression profile of cancer marker genes in HepG2 cells treated with different concentrations of a new indolin3one from Pseudomonas aeruginosa
作者: Lucas Milanez BenicioAne Stefano SimionatoCláudio Roberto NovelloJeconias Rocha GuimarãesIngrid FelicidadeAdmilton Gonçalves de OliveiraJoão Carlos Palazzo de MelloMário Sérgio MantovaniAndreas Lazaros ChryssafidisGaldino AndradeIlce Mara de Syllos ColusMarcelo Tempesta de Oliveira
刊名: Scientific Reports, 2018, Vol.8 (1), pp.1-9
来源数据库: Directory of Open Access Journals
DOI: 10.1038/s41598-018-30893-w
原始语种摘要: Abstract The present study tested the effects of a newly identified indolin-3-one compound (compound 1, produced by Pseudomonas aeruginosa, on HepG2 cells. The MTT assays demonstrated decreased metabolic activities in HepG2 cells treated with compound 1, with dose- and time-dependent intensifying effect, starting at a concentration of 40u2009µM. The IC50 after 24, 48, 72, and 96u2009h treatments were 41.35, 52.7, 92.79 and 66.65u2009μM of compound 1, respectively. Below 80u2009µM, no significative damage on erythrocytes membranes was observed by the hemolytic assays. The RT-qPCR revealed that the compound modulated key genes involved in carcinogenesis process, indicating possible indolin-3-one mechanisms of action. The data showed that gene expression alterations promoted by compound 1,...
全文获取路径: DOAJ  (合作)
影响因子:2.927 (2012)

  • expression 表示
  • concentration 浓度
  • marker 标试器
  • observed 观察到的
  • demonstrated 探明的储量
  • compound 复合物
  • significative 有意义的
  • cells 麻风细胞
  • treated 加工的
  • addition 添加