Newcastle Disease Virus infection study on duck and chicken in Subang district
作者: Aprizal PanusS. SetiyaningsihN.L.P.I. Mayasari
作者单位: 1Institute of Veterinary Subang, FKH IPB
刊名: Jurnal Ilmu Ternak dan Veteriner, 2015, Vol.20 (2), pp.134-147
来源数据库: Directory of Open Access Journals
DOI: 10.14334/jitv.v20i2.1168
关键词: Newcastle DiseaseRRT-PCR DetectionDetection of VirulenceAntigenic DiversityAntibody
原始语种摘要: The objectives of this research were to study Newcastle Disease Virus (NDV) infection in Subang area and to examine the diversity of the circulating NDV. Swabs of cloacal and oropharynx, and serum were sampled from total of 393 chickens and 149 ducks in backyard farms and live bird markets located in 10 subdistricts. Screening of NDV in pool of 5-7 samples by real-time Reverse-Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M) showed 19/67 (28.3%) cloacal and 8/67 (11.9%) pharyngeal pools of chicken samples; 18/67 (26.9%) of the pools excreted virus via cloaca and oropharynx, while the duck pools of 8/30 (26.7%) shed virus from cloaca. Virus isolation attempted on individual sample from positive pools yielded 18 isolates which the majority of the isolates showed homogeneous...
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  • chicken 小鸡
  • cloacal 阴沟的
  • velogenic velogenic
  • isolates 单离体
  • antigenic 抗原的
  • virus 病毒
  • virulent 毒性的
  • vaccinated 接种疫苗的
  • sampled 抽[采,取]样
  • oropharynx 口咽