A comparative study on fungal laccases immobilized on chitosan
作者: José Hilton Bernardino de AraújoVinicius Oliveira UemuraFlavio Faria de MoraesAneli de Melo BarbosaGisella Maria Zanin
刊名: Brazilian Archives of Biology and Technology, 2005, Vol.48 (spe), pp.1-6
来源数据库: Directory of Open Access Journals
DOI: 10.1590/S1516-89132005000400001
关键词: LaccasesImmobilizationChitosan
原始语种摘要: The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement of white wines by the degradation...
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  • chitosan 壳聚糖
  • granulometry 粒度测量
  • activity 活度
  • degradation 减嚣夷酌
  • enzymatic 酶催的
  • laccase 漆酶
  • foram 有孔虫
  • adsorption 吸附
  • phenoloxidase 酚酶
  • conditions 条件式