Impact of Autophagy Inhibition at Different Stages on Cytotoxic Effect of Autophagy Inducer in Glioblastoma Cells
作者: Chenguang LiYaohua LiuHuailei LiuWeiguang ZhangChen ShenKenKa ChoXin ChenFei PengYunke BiXu HouZhuowen YangZhixing ZhengKaikai WangXiaoxiong WangJiakang ZhangChen ZhongHuichao ZouXinjian ZhangShiguang Zhao
刊名: Cellular Physiology and Biochemistry, 2015, Vol.35 (4), pp.1303-1316
来源数据库: Directory of Open Access Journals
DOI: 10.1159/000373952
关键词: Autophagy inhibitionGlioblastoma multiformeApoptosisAutophagic vacuoleAutophagy inducer
原始语种摘要: Background/Aims: Glioblastoma multiforme (GBM) is the most malignant primary brain tumor with a poor prognosis. Combination treatment of autophagy inducer and autophagy inhibitor may be a feasible solution to improve the therapeutic effects. However, the correlation between them is unclear. The purpose of this study was to investigate the effect of autophagy inhibition at different stages on cytotoxicity of autophagy inducers in glioblastoma cells. Methods: Autophagy inhibition at early stage was achieved by 3-methyladenine (3-MA) or Beclin 1 shRNA. Autophagy inhibition at late stage was achieved by chloroquine (CQ) or Rab7 shRNA. Cell viability was assessed by MTT assay. Autophagy was measured using transmission electron microscopy and western blot. Apoptosis was measured using western...
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  • autophagy 自体消瘦
  • cytotoxicity 细胞毒性
  • stage 
  • synergistic 协同的
  • prognosis 预报
  • knockdown 击倒
  • therapeutic 治疗的
  • inhibition 抑制
  • chloroquine 氯奎
  • depletion 矿量递减