Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants
作者: Zheng-Yi WeiYu-Ying ZhangYun-Peng WangMing-Xia FanXiao-Fang ZhongNuo XuFeng LinShao-Chen Xing
作者单位: 1gronomy College, Shenyang Agricultural University, No. 120 Dongling Rd., Shenhe Distirct, Shenyang 110866, China
2Laboratory of Plant Bioreactor and Genetics Engineering, Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai St., Changchun 130033, China
3Laboratory of Plant Bioreactor and Genetics Engineering, Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Ins
刊名: International Journal of Molecular Sciences, 2016, Vol.17 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/ijms17050624
关键词: ChymosinRenninTobaccoMilk coagulationTransformation
原始语种摘要: Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome under control of the viral 35S cauliflower mosaic promoter. The integration and transcription of the foreign gene were confirmed with Southern blotting and reverse transcription PCR (RT-PCR) analyses, respectively. Immunoblotting analyses were performed to demonstrate expression of chymosin, and the expression level was quantified by enzyme-linked immunosorbent assay (ELISA). The results indicated...
全文获取路径: DOAJ  (合作)
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影响因子:2.464 (2012)

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关键词翻译
关键词翻译
  • chymosin 凝乳酶
  • alternative 可选择的
  • bovine 牛的
  • expression 表示
  • tobacco 烟草
  • confirmed 已确认的
  • coagulation 凝聚
  • expressed 榨出的
  • transcription 地名转写
  • bioactivity 生物活性