Effect of MRE11 loss on PARP-inhibitor sensitivity in endometrial cancer in vitro.
作者: Romana KoppensteinerEleftherios P SamartzisAurelia NoskeAdriana von TeichmanIoannis DedesMyriam GwerderPatrick ImeschKristian IkenbergHolger MochDaniel FinkManuel StuckiKonstantin J Dedes
刊名: PLoS ONE, 2017, Vol.9 (6)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0100041
原始语种摘要: To evaluate the frequency of MRE11/RAD50/NBS1 (MRN)-complex loss of protein expression in endometrial cancers (EC) and to determine whether loss of MRE11 renders the cancer cells sensitive to Poly(ADP-ribose) polymerase (PARP)-inhibitory treatment.MRN expression was examined in 521 samples of endometrial carcinomas and in 10 cancer cell lines. A putative mutation hotspot in the form of an intronic poly(T) allele in MRE11 was sequenced in selected cases (n = 26). Sensitivity to the PARP-inhibitor, BMN673 was tested in colony formation assays before and after MRE11 silencing using siRNA. Homologous recombination (HR) DNA repair was evaluated by RAD51-foci formation assay upon irradiation and drug treatment.Loss of MRE11 protein was found in 30.7% of EC tumours and significantly associated...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • inhibitor 抑制剂
  • endometrial 子宫内膜的
  • expression 表示
  • sensitivity 灵敏度
  • evaluate 求...的值
  • vitro 体外
  • formation 建造
  • poly 
  • protein 蛋白质
  • defining 定义