Targeted ubiquitination and degradation of G-protein-coupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex.
作者: Ziyan WuYuejun ChenTong YangQinqin GaoMan YuanLan Ma
刊名: PLoS ONE, 2017, Vol.7 (8)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0043997
原始语种摘要: The G protein-coupled receptor kinases (GRKs) phosphorylate agonist occupied G protein-coupled receptors (GPCRs) and desensitize GPCR-mediated signaling. Recent studies indicate they also function non-catalytically via interaction with other proteins. In this study, a proteomic approach was used to screen interacting proteins of GRK5 in MDA-MB-231 cells and HUVEC cells. Mass spectrometry analysis reveals several proteins in the GRK5 immunocomplex including damaged DNA-binding protein 1 (DDB1), an adaptor subunit of the CUL4-ROC1 E3 ubiquitin ligase complex. Co-immunoprecipitation experiments confirmed the association of GRK5 with DDB1-CUL4 complex, and reveal that DDB1 acts as an adapter to link GRK5 to CUL4 to form the complex. Overexpression of DDB1 promoted, whereas knockdown of DDB1...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • ligase 连接酶
  • complex 超群
  • protein 蛋白质
  • coupled 耦合
  • degradation 减嚣夷酌
  • interacting 相克的
  • knockdown 击倒
  • receptor 接受体
  • several 各自
  • confirmed 已确认的