Role of Glucokinase in the Subcellular Localization of Glucokinase Regulatory Protein
作者: Ling JinTingting GuoZhixin LiZhen LeiHui LiYiqing MaoXi WangNa ZhouYizhuang ZhangRuobi HuXuehui ZhangGang NiuDavid M. IrwinHuanran Tan
作者单位: 1epartment of Pharmacology, Peking University, Health Science Center, Beijing 100191, China
2Department of Pharmacology, Peking University, Health Science Center, Beijing 100191, China
3Department of Integrated Traditional Chinese and Western Medicine, Peking University, Health Science Center, Beijing 100191, China
4Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China
5Department of Pharmac
刊名: International Journal of Molecular Sciences, 2015, Vol.16 (4), pp.7377-7393
来源数据库: Directory of Open Access Journals
DOI: 10.3390/ijms16047377
关键词: Protein–protein interactionGlucokinase regulatory proteinGlucokinaseSub-cellular localizationGlucose metabolism
原始语种摘要: Glucokinase (GCK) is the rate-limiting enzyme of liver glucose metabolism. Through protein-protein interactions, glucokinase regulatory protein (GCKR) post-transcriptionally regulates GCK function in the liver, and causes its nuclear localization. However the role of GCK in regulating GCKR localization is unknown. In the present study, using in vitro and in vivo models, we examined the levels of GCK and GCKR, and their subcellular localization. We found that total cellular levels of GCKR did not vary in the in vivo models, but its subcellular localization did. In animals with normal levels of GCK, GCKR is mainly localized to the nuclei of hepatocytes. In seven-day old rats and liver-specific Gck gene knockout mice (animals that lack or have reduced levels of GCK protein), GCKR was found...
全文获取路径: DOAJ  (合作)
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影响因子:2.464 (2012)

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关键词翻译
关键词翻译
  • protein 蛋白质
  • regulatory 控制的
  • glucokinase 葡糖激酶
  • localization 定位
  • regulating 蝶的
  • cellular 细胞状的
  • metabolism 代谢
  • knockout 出模器
  • interaction 相互酌
  • reduced 减缩的