Sensitive Detection of 8-Nitroguanine in DNA by Chemical Derivatization Coupled with Online Solid-Phase Extraction LC-MS/MS
作者: Chiung-Wen HuYuan-Jhe ChangJian-Lian ChenYu-Wen HsuMu-Rong Chao
作者单位: 1Department of Public Health, Chung Shan Medical University, Taichung 402, Taiwan
2Department of Occupational Safety and Health, Chung Shan Medical University, Taichung 402, Taiwan
3School of Pharmacy, China Medical University, Taichung 404, Taiwan
刊名: Molecules, 2018, Vol.23 (3)
来源数据库: Directory of Open Access Journals
DOI: 10.3390/molecules23030605
关键词: Online solid-phase extractionLC-MS/MSPeroxynitriteNitrated DNA lesionDerivatizationIsotope-dilution
原始语种摘要: 8-Nitroguanine (8-nitroG) is a major mutagenic nucleobase lesion generated by peroxynitrite during inflammation and has been used as a potential biomarker to evaluate inflammation-related carcinogenesis. Here, we present an online solid-phase extraction (SPE) LC-MS/MS method with 6-methoxy-2-naphthyl glyoxal hydrate (MTNG) derivatization for a sensitive and precise measurement of 8-nitroG in DNA. Derivatization optimization revealed that an excess of MTNG is required to achieve complete derivatization in DNA hydrolysates (MTNG: 8-nitroG molar ratio of 3740:1). The use of online SPE effectively avoided ion-source contamination from derivatization reagent by washing away all unreacted MTNG before column chromatography and the ionization process in mass spectrometry. With the use of...
全文获取路径: DOAJ  (合作)
影响因子:2.428 (2012)

  • derivatization 衍生作用
  • extraction 提取
  • satisfactory 满意的
  • phase 相位
  • naphthyl 萘基
  • ionization 电离化
  • inflammation 炎症
  • measurement 测量
  • intraday 一天内的
  • dilution 稀度