Comparative proteomic analysis of Methanothermobacter themautotrophicus ΔH in pure culture and in co-culture with a butyrate-oxidizing bacterium.
作者: Miho EnokiNaoya ShinzatoHiroaki SatoKohei NakamuraYoichi Kamagata
刊名: PLoS ONE, 2017, Vol.6 (8)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0024309
原始语种摘要: To understand the physiological basis of methanogenic archaea living on interspecies H(2) transfer, the protein expression of a hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus strain ΔH, was investigated in both pure culture and syntrophic coculture with an anaerobic butyrate oxidizer Syntrophothermus lipocalidus strain TGB-C1 as an H(2) supplier. Comparative proteomic analysis showed that global protein expression of methanogen cells in the model coculture was substantially different from that of pure cultured cells. In brief, in syntrophic coculture, although methanogenesis-driven energy generation appeared to be maintained by shifting the pathway to the alternative methyl coenzyme M reductase isozyme I and cofactor F(420)-dependent process, the machinery proteins...
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影响因子:3.73 (2012)

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关键词翻译
关键词翻译
  • methanogen 产甲烷生物
  • syntrophic 合营生活
  • oxidizing 氧化
  • culture 
  • anaerobic 嫌气的
  • analysis 分析
  • conditions 条件式
  • restrained 抑制
  • protein 蛋白质
  • bacterium 细菌