Effect of Mannitol on Hyaluronic Acid Stability in Two in Vitro Models of Oxidative Stress
作者: Marguerite RinaudoBernard LardyLaurent GrangeThierry Conrozier
作者单位: 1Biomaterials Applications, 6 rue Lesdiguières, 38000 Grenoble, France
2Université Joseph Fourier, GREPI AGIM FRE 3405 CNRS, 38000 Grenoble, France
3Service de Rhumatologie, Centre hospitalier Universitaire A.Michallon, 38000 Grenoble, France
4Service de Rhumatologie, Centre hospitalier de Belfort-Montbéliard, 90000 Belfort, France
刊名: Polymers, 2014, Vol.6 (7), pp.1948-1957
来源数据库: Directory of Open Access Journals
DOI: 10.3390/polym6071948
关键词: Hyaluronic acidOsteoarthritisViscosupplementationAnti-oxidantMannitolXanthine oxidaseHydrogen peroxideIn vitro
原始语种摘要: In this paper, we propose the evaluation of the mannitol’s ability to reduce hyaluronic acid (HA) degradation using two different models of oxidative stress. Firstly, a solution of hyaluronan and a solution of the same HA including mannitol in PBS buffer were submitted to an oxidative stress generated by the addition of xanthine + xanthine oxidase generating oxygen free radicals. Different enzyme concentrations were used and the HA properties were studied after 24 h of contact at ambient temperature. Decreases of the viscosity of the solution were assessed by rheometry (viscous and elastic module) and that of HA molecular weight was determined by steric exclusion chromatography. Rheologic behavior was assessed on identical HA solutions subjected to another model of oxidative stress...
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  • degradation 减嚣夷酌
  • solution 溶液
  • concentrations 浓聚物
  • oxygen 
  • peroxide 过氧化
  • studied 学习
  • mannitol 甘露糖醇
  • reduce 减少
  • xanthine 黄质
  • increase 增加