An evolutionarily conserved phosphatidate phosphatase maintains lipid droplet number and endoplasmic reticulum morphology but not nuclear morphology
作者: Anoop Narayana PillaiSushmita ShuklaAbdur Rahaman
作者单位: 1School of Biological Sciences, National Institute of Science Education and Research (NISER)-Bhubaneswar, HBNI, P.O. Jatni, Khurda 752050, Odisha, India
刊名: Biology Open, 2017, Vol.6 (11), pp.1629-1643
来源数据库: Directory of Open Access Journals
DOI: 10.1242/bio.028233
关键词: Phosphatidic acid hydrolaseLipinTetrahymena thermophilaLipid dropletNuclear membrane expansionEndoplasmic reticulum
原始语种摘要: Phosphatidic acid phosphatases are involved in the biosynthesis of phospholipids and triacylglycerol, and also act as transcriptional regulators. Studies to ascertain their role in lipid metabolism and membrane biogenesis are restricted to Opisthokonta and Archaeplastida. Here, we report the role of phosphatidate phosphatase (PAH) in Tetrahymena thermophila, belonging to the Alveolata clade. We identified two PAH homologs in Tetrahymena, TtPAH1 and TtPAH2. Loss of function of TtPAH1 results in reduced lipid droplet number and an increase in endoplasmic reticulum (ER) content. It also results in more ER sheet structure as compared to wild-type Tetrahymena. Surprisingly, we did not observe a visible defect in the nuclear morphology of the ΔTtpah1 mutant. TtPAH1 rescued all known defects in...
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  • phosphatidate 磷脂酸
  • droplet 液滴
  • nuclear 原子核的
  • phosphatase 磷酸酶
  • morphology 形态学
  • homologous 同结构型的
  • eukaryotic 真核的
  • biogenesis 生源说
  • lipid 脂质
  • reticulum 蜂巢胃