Effects of exogenous recombinant human bone morphogenic protein-7 on the corneal epithelial mesenchymal transition and fibrosis
作者: Jin Kwon ChungShin Ae ParkHee Sun HwangKwang Sung KimYang Je ChoYong Sung YouYoung Sik KimJu Woong JangSung Jin Lee
作者单位: 1epartment of Ophthalmology, Soonchunhyang University College of Medicine, Soonchunhyang University Seoul Hospital, Seoul 04401, Korea.
2Eyegene Inc., Seoul 07528, Korea
3Nune Eye Hospital, Seoul 06198, Korea
4Cellumed. Co., Ltd., Seoul 08501, Korea
5Department of Ophthalmology, Soonchunhyang University College of Medicine, Soonchunhyang University Seoul Hospital, Seo
刊名: International Journal of Ophthalmology, 2017, Vol.10 (3), pp.329-335
来源数据库: Directory of Open Access Journals
DOI: 10.18240/ijo.2017.03.01
关键词: Bone morphogenic protein-7Corneal fibrosisEpithelial mesenchymal transitionMyodifferentiationTransforming growth factor-βTransforming growth factor-β
原始语种摘要: AIM: To evaluate the effect of exogenous recombinant human bone morphogenic protein-7 (rhBMP-7) on transforming growth factor-β (TGF-β)-induced epithelial mesenchymal cell transition (EMT) and assessed its antifibrotic effect via topical application. METHODS: The cytotoxic effect of rhBMP-7 was evaluated and the EMT of human corneal epithelial cells (HECEs) was induced by TGF-β. HECEs were then cultured in the presence of rhBMP-7 and/or hyaluronic acid (HA). EMT markers, fibronectin, E-cadherin, α-smooth muscle actin (α-SMA), and matrix metaloproteinase-9 (MMP-9), were evaluated. The level of corneal fibrosis and the reepithelization rate were evaluated using a rabbit keratectomy model. Expression of α-SMA in keratocytes were quantified following treatment with different concentrations of...
全文获取路径: DOAJ  (合作)
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影响因子:0.119 (2012)

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关键词翻译
关键词翻译
  • corneal 角膜的
  • fibrosis 纤维化
  • protein 蛋白质
  • mesenchymal 间叶细胞的
  • epithelial 上皮的
  • keratocyte 角膜细胞
  • transition 转移
  • keratectomy 角膜切除术
  • recombinant 重组体
  • exogenous 外源的