Periodic Mechanical Stress Induces Extracellular Matrix Expression and Migration of Rat Nucleus Pulposus Cells Through Src-GIT1-ERK1/2 Signaling Pathway
作者: Gongming GaoHaibo LiYongjing HuangJianjian YinYuqing JiangNanwei XuDong ZhouLuming NongKewei Ren
刊名: Cellular Physiology and Biochemistry, 2018, Vol.50 (4), pp.1510-1521
来源数据库: Directory of Open Access Journals
DOI: 10.1159/000494650
关键词: Periodic mechanical stressExtracellular matrixNucleus pulposus cellsSrcGIT1ERK1/2
原始语种摘要: Background/Aims: Periodic mechanical stress has been shown to promote extracellular matrix (ECM) synthesis and cell migration of nucleus pulposus (NP) cells, however, the mechanisms need to be fully elucidated. The present study aimed to investigate the signal transduction pathway in the regulation of NP cells under periodic mechanical stress. Methods: Primary rat NP cells were isolated and seeded on glass slides, and then treated in our self-developed periodic stress field culture system. To further explore the mechanisms, data were analyzed by scratch-healing assay, quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis, western blotting, and co-immunoprecipitation assay. Results: Under periodic mechanical stress, the mRNA expression of ECM collagen 2A1 (Col2A1)...
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关键词翻译
关键词翻译
  • GIT Generic Intelligent center
  • ECM Effective Complex Modules
  • mechanical 机械的
  • migration 迁移
  • periodic 周期的
  • cells 麻风细胞
  • stress 应力
  • synthesis 合成
  • between 在中间
  • promote 助长