Lipid raft-dependent FcepsilonRI ubiquitination regulates receptor endocytosis through the action of ubiquitin binding adaptors.
作者: Rosa MolfettaFrancesca GasparriniGiovanna PeruzziLaura VianMario PiccoliLuigi FratiAngela SantoniRossella Paolini
刊名: PLoS ONE, 2017, Vol.4 (5)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0005604
原始语种摘要: The best characterized role for ubiquitination of membrane receptors is to negatively regulate signaling by targeting receptors for lysosomal degradation. The high affinity receptor for IgE (FcepsilonRI) expressed on mast cells and basophils is rapidly ubiquitinated upon antigen stimulation. However, the nature and the role of this covalent modification are still largelly unknown. Here, we show that FcepsilonRI subunits are preferentially ubiquitinated at multiple sites upon stimulation, and provide evidence for a role of ubiquitin as an internalization signal: under conditions of impaired receptor ubiquitination a decrease of receptor entry is observed by FACS analysis and fluorescence microscopy. We also used biochemical approaches combined with fluorescence microscopy, to demonstrate...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • receptor 接受体
  • endocytosis 内吞
  • lysosomes 溶菌体
  • sorting 分选酌
  • affinity 亲和力
  • stimulation 激励
  • lipid 脂质
  • interference 干涉
  • endocytic 内吞作用的
  • compartment 隔间