Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis.
作者: Yong QiXiaolu XiongXile WangChangsong DuanYinjun JiaJun JiaoWenping GongBohai Wen
刊名: PLoS ONE, 2017, Vol.8 (7)
来源数据库: Directory of Open Access Journals
DOI: 10.1371/journal.pone.0070440
原始语种摘要: BACKGROUND: Rickettsia heilongjiangensis, the agent of Far-Eastern spotted fever (FESF), is an obligate intracellular bacterium. The surface-exposed proteins (SEPs) of rickettsiae are involved in rickettsial adherence to and invasion of host cells, intracellular bacterial growth, and/or interaction with immune cells. They are also potential molecular candidates for the development of diagnostic reagents and vaccines against rickettsiosis. METHODS: R. heilongjiangensis SEPs were identified by biotin-streptavidin affinity purification and 2D electrophoreses coupled with ESI-MS/MS. Recombinant SEPs were probed with various sera to analyze their serological characteristics using a protein microarray and an enzyme-linked immune sorbent assay (ELISA). RESULTS: Twenty-five SEPs were identified,...
全文获取路径: DOAJ  (合作)
影响因子:3.73 (2012)

  • proteins 蛋白质
  • analysis 分析
  • potential 
  • bacterial 细菌的
  • involved 涉及
  • exposed 被辐照的
  • rickettsiosis 立克次氏体病
  • vaccines 疫苗
  • major 知的
  • spotted 有斑点的