High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
作者: Dirk PonselJulia NeugebauerKathrin Ladetzki-BaehsKathrin Tissot
刊名: Molecules, 2011, Vol.16 (5), pp.3675
来源数据库: Directory of Open Access Journals
关键词: phage displaylibrary
原始语种摘要: Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library. Concurrent evolution of the libraries has allowed display and selection technologies to reveal their full potential. They come in different flavors, from naïve to fully synthetic and differ in terms of size, quality, method of preparation, framework and CDR composition. Early on, the focus has mainly been on affinities and thus on library size and diversity. Subsequently, the increased awareness of developability and cost of goods as important success factors has spurred efforts to generate libraries with improved biophysical properties and...
全文获取路径: DOAJ  (合作)
影响因子:2.428 (2012)

  • quality 品质
  • improved 改进
  • recently 近来
  • selection 选择
  • spurred 具距的
  • library 图书馆
  • fully 充分
  • developed 开发
  • their 他们的
  • focus 焦点