Incorporation of osteoblasts (MG63) into 3D nanofibre matrices by simultaneous electrospinning and spraying in bone tissue engineering
作者: JRJ PalettaF MackH SchenderleinC TheisenJ SchmittJH WendorffS AgarwalS Fuchs-WinkelmannMD Schofer
刊名: European Cells and Materials (ECM), 2011, Vol.21 , pp.384
来源数据库: Directory of Open Access Journals
关键词: Electrospinningelectrospraying3D nanofibre matricesosteoblastsgene expressionbone tissue engineering
原始语种摘要: Nanofibre scaffolds are suitable tools for bone tissue engineering. Mimicking the extracellular matrix, they allow for cell growth and differentiation. However, in large 3D scaffolds, uniform cell colonisation presents an unsolved problem. Our aim was to design and analyse a method of colonising nanofibre scaffolds, combining electrospinning of fibres and electrospraying of cells, to determine its impact on cell survival, growth, and gene expression. The osteoblast-like cell line MG63 was suspended in medium and electrosprayed into growing scaffolds of poly-(l-lactic acid) (PLLA) or PLLA/Col-I blend nanofibres. Fluorescein diacetate (FDA) staining was used to determine survival and growth over a 22 d culture period. Expression of osteocalcin (OC) and type I collagen (Col-I) genes was...
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影响因子:4.558 (2012)

  • simultaneous 同时的
  • spraying 喷涂
  • suitable 合适的
  • suspended 悬浮
  • engineering 工程
  • polymer 聚合物
  • solution 溶液
  • osteoblast 成骨细胞
  • impact 冲击碰撞
  • survival 生存