A 3D in vitro bone organ model using human progenitor cells
作者: A PapadimitropoulosA ScherberichS GüvenN TheilgaardHJA CrooijmansF SantiniK SchefflerA ZalloneI Martin
刊名: European Cells and Materials (ECM), 2011, Vol.21 , pp.445
来源数据库: Directory of Open Access Journals
关键词: Perfusion bioreactorhuman stem cells- population regulationtissue engineeringregenerative medicinebone remodellingnon invasive toolsmulti-cell co-culture
原始语种摘要: Three-dimensional (3D) organotypic culture models based on human cells may reduce the use of complex and costly animal models, while gaining clinical relevance. This study aimed at developing a 3D osteoblastic-osteoclastic-endothelial cell co-culture system, as an in vitro model to mimic the process of bone turnover. Osteoprogenitor and endothelial lineage cells were isolated from the stromal vascular fraction (SVF) of human adipose tissue, whereas CD14+ osteoclast progenitors were derived from human peripheral blood. Cells were co-cultured within 3D porous ceramic scaffolds using a perfusion-based bioreactor device, in the presence of typical osteoclastogenic factors. After 3 weeks, the scaffolds contained cells with endothelial (2.0 ±0.3%), pre/osteoclastic (14.0 ±1.4%) and...
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影响因子:4.558 (2012)

  • osteoclastic 折骨的
  • cells 麻风细胞
  • resorption 再吸收
  • human 人的
  • vitro 体外
  • functional 功能的
  • matrix 石基
  • tartrate 酒石酸盐
  • culture 
  • lineage 血统