Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stoichiometry
作者: Grigsby Iwen Zhang WeiJohnson Jolene Fogarty Keir Chen YanRawson Jonathan Crosby Aaron Mueller Joachim Mansky Louis
刊名: Retrovirology, 2010, Vol.7 (1), pp.75
来源数据库: Directory of Open Access Journals
原始语种摘要: Abstract Background Human T-lymphotropic virus type 1 (HTLV-1) is an important human retrovirus that is a cause of adult T-cell leukemia/lymphoma. While an important human pathogen, the details regarding virus replication cycle, including the nature of HTLV-1 particles, remain largely unknown due to the difficulties in propagating the virus in tissue culture. In this study, we created a codon-optimized HTLV-1 Gag fused to an EYFP reporter as a model system to quantitatively analyze HTLV-1 particles released from producer cells. Results The codon-optimized Gag led to a dramatic and highly robust level of Gag expression as well as virus-like particle (VLP) production. The robust level of particle production overcomes previous technical difficulties with authentic particles and allowed for...
全文获取路径: DOAJ  (合作)
影响因子:5.657 (2012)

  • particle 颗粒
  • novel 长篇小说
  • morphology 形态学
  • analysis 分析
  • virus 病毒
  • quantitatively 定量的
  • observed 观察到的
  • stoichiometry 化学计量学
  • likely 大概
  • reporter 报告生成程序