Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia
作者: Stevanović JevrosimaStanimirović Z.Dimitrijević V.Stojić V.Fratrić NatalijaLazarević M.
刊名: Acta Veterinaria, 2009, Vol.59 (5-6), pp.621
来源数据库: Directory of Open Access Journals
关键词: cattlemicrosatellitespedigreepolymorphismSerbia
原始语种摘要: The polymorphism of 11 microsatellite DNA markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) was analyzed in order to assess the usefulness of these markers for pedigree verification in the Serbian population of Simmental cattle. A total of 86 animals were involved in the study. Genomic DNA was extracted from sperm and blood and amplified in a multiplex PCR using primers from the commercial 'StockMarks for Cattle® Bovine Genotyping Kit'. Analyses were done with automated DNA sizing technology. A total of 92 alleles were detected. The mean number of alleles (nA) per locus was 8.364, the mean value of polymorphism information content (PIC) was 0.73, and the mean frequency of the most frequent allele (FNA) was 0.372. The most polymorphic...
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  • pedigree 谱系图
  • polymorphism 多晶形
  • alleles 对立形质
  • cattle 
  • usefulness 有效
  • markers 时标
  • locus 轨迹
  • population 母体
  • PIC Particle In Cell method
  • combined 组合