Simultaneous determination of ofloxacin and ornidazole in solid dosage form by RP-HPLC and HPTLC techniques
作者: Puranik ManishaBhawsar D Rathi PrachiYeole P
刊名: Indian Journal of Pharmaceutical Sciences, 2010, Vol.72 (4), pp.513
来源数据库: Directory of Open Access Journals
关键词: RP-HPLCHPTLCofloxacinornidazolemarketed formulation.
原始语种摘要: The objective of this work was to develop and validate simple, rapid and accurate chromatographic methods for simultaneous determination of ofloxacin and ornidazole in solid dosage form. The first method was based on reversed phase high performance liquid chromatography, on Intersil C 18 column (250 mm, 4.6 i.d.), using acetonitrile:methanol: 0.025M phosphate buffer, pH 3.0 (30:10:60 % v/v/v) as the mobile phase, at a flow rate of 1 ml/min at ambient temperature. Quantification was achieved with UV detection at 318 nm over a concentration range of 2-40 μg/ml for ofloxacin and 5-100 μg/ml for ornidazole. The mean retention time of ofloxacin and ornidazole was found to be 4.04 min and 5.83 min, 6.77 min (isomers), respectively. The amount of ofloxacin and ornidazole estimated as...
全文获取路径: DOAJ  (合作)
影响因子:0.338 (2012)

  • ornidazole 奥硝唑
  • ofloxacin 氧氟沙星
  • determination 测定
  • dosage 放射剂量
  • recoveries 追回款
  • found 创立
  • tablets 平片体
  • reversed 倒转的
  • percentage 百分数
  • concentration 浓度